Microscopy

Biophysical methods of analysis. Advanced fluorescence microscopy has revealed molecular insights into the spatiotemporal dynamics of biological processes that could not have been obtained by any other means. In the Laboratory for Cellular Biophysics we develop and apply methods of analysis based on fluorescence lifetime imaging microscopy (FLIM) and fluorescence fluctuation spectroscopy (FFS) to quantify protein interaction, aggregation, binding kinetics and diffusion in live cells. This includes the following:

  1. Fluorescence lifetime imaging microscopy (FLIM) – FRET biosensor detection
  2. Fluorescence correlation spectroscopy (FCS) – protein diffusion and concentration
  3. Moment based brightness analysis (NB, PCH) – protein oligomerisation
  4. Spatiotemporal correlation spectroscopy (STICS, TICS, RICS) – protein binding dynamics
  5. Pair correlation function analysis (pCF, pCOMB) – protein transport and accessibility